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Methylmercury intoxication and histochemical demonstration of NADPH-diaphorase activity in the striate cortex of adult cats BJMBR
Oliveira,R.B.; Gomes-Leal,W.; do-Nascimento,J.L.M.; Picanço-Diniz,C.W..
The effects of methylmercury (MeHg) on histochemical demonstration of the NADPH-diaphorase (NADPH-d) activity in the striate cortex were studied in 4 adult cats. Two animals were used as control. The contaminated animals received 50 ml milk containing 0.42 µg MeHg and 100 g fish containing 0.03 µg MeHg daily for 2 months. The level of MeHg in area 17 of intoxicated animals was 3.2 µg/g wet weight brain tissue. Two cats were perfused 24 h after the last dose (group 1) and the other animals were perfused 6 months later (group 2). After microtomy, sections were processed for NADPHd histochemistry procedures using the malic enzyme method. Dendritic branch counts were performed from camera lucida drawings for control and intoxicated animals (N = 80). Average,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: NADPH-dehydrogenase; Visual cortex; Neurotoxicity; Neuropil; Mercury.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900009
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NADPH-diaphorase activity in area 17 of the squirrel monkey visual cortex: neuropil pattern, cell morphology and laminar distribution BJMBR
Franca,J.G.; do-Nascimento,J.L.M.; Picanço-Diniz,C.W.; Quaresma,J.A.S.; Silva,A.L.C..
We studied the distribution of NADPH-diaphorase activity in the visual cortex of normal adult New World monkeys (Saimiri sciureus) using the malic enzyme "indirect" method. NADPH-diaphorase neuropil activity had a heterogeneous distribution. In coronal sections, it had a clear laminar pattern that was coincident with Nissl-stained layers. In tangential sections, we observed blobs in supragranular layers of V1 and stripes throughout the entire V2. We quantified and compared the tangential distribution of NADPH-diaphorase and cytochrome oxidase blobs in adjacent sections of the supragranular layers of V1. Although their spatial distributions were rather similar, the two enzymes did not always overlap. The histochemical reaction also revealed two different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide synthase; Neocortex; NADPH-diaphorase; Cytochrome oxidase; Primates.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000900008
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